LOCUS       40924_2977       11068 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pA1-Agc-bgyg-3'N DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Ikeda Y, Ito K, Izumi Y, Shinomura T.
  TITLE     A candidate enhancer element responsible for high-level expression 
            of the aggrecan gene in chondrocytes
  JOURNAL   J. Biochem. 156 (1), 21-28 (2014)
  PUBMED    24554731
REFERENCE   2  (bases 1 to 11068)
  AUTHORS   Ikeda Y, Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and 
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan URL :http://www.tmd.ac.jp/
REFERENCE   3  (bases 1 to 11068)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11068)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2014"; volume: "156"; issue: "1"; pages: "21-28"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 
            113-8549, Japan URL :http://www.tmd.ac.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_binding    207..565
                     /label=SOX9 binding site
                     /bound_moiety="SOX9"
                     /note="A1"
     regulatory      704..1237
                     /gene="bgyg"
                     /label=rat Agc1 promoter sequence
                     /note="rat Agc1 promoter sequence"
                     /regulatory_class="promoter"
     exon            1238..1618
                     /gene="bgyg"
                     /note="rat Agc1 exon 1 sequence"
     intron          1619..2673
                     /gene="bgyg"
     CDS             2855..5896
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             5912..6946
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     promoter        complement(7937..7955)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7973..7989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7997..8013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8021..8051)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8066..8087)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8375..8963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9137..9994)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9995..10099)
                     /label=AmpR promoter
     rep_origin      10433..10888
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     11029..11045
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcgaattc gagctcggta cccggggatc ctctagagtc gacagtttct tttcactgac
       61 tgtacaaagg agcccccgtg ctccctgccc cactcattcc gtcctccaca accactgggg
      121 tgacacccaa accaacagcc acaggtaaac ggtgtctctc cctggcatgc aaagttaatt
      181 acacaatccc agagaagtga cccgggtcca gaatggaagg gaccaaggtg ccagggatga
      241 ccaagccctc aagaatcccc ctagaagctc ctccctggag acacccttcc ccagaatagg
      301 cagagagggc tagaaacacc catataaaca gcgcatccac aaaaccccac tgtcccaagg
      361 ctttcgtccc tcagcctttg ctatttttat ctggagatcg agaagggggt tgccaggcag
      421 ggccacgccg ctgtttatgt ctgaaatttg aaaagatgca cagtgtactt gggagaaggc
      481 gttgcaggga aaggaatttc agagaatttc aaacctggca gggtgaatag gtgccttgtg
      541 ttcaggctta cgactctgga aaagctcttc aacccatggc acgaagaggg gccacggacg
      601 ggtgcaaacg cccacgtggg agatttggga tggatggaga ctcagttcac ctggctgagg
      661 ggaatcaact gatctcagac tgggggtgct ttacgaggaa gtgggtaccc cacccccaaa
      721 actttccaac actgttctgt aagggttctt tcaagtagca ttcccagggt agggggtcgt
      781 cctgcgcgat cgcatctttc tgggtctcag ctgcaaatcg cctccaggta tgggggggga
      841 accacccctc cccgcggcca gggcctgtca ttctgacagc aggagtcggg tgctttcggc
      901 actctgcggg cacagccctc cggggcctcc tggactgcgg agcttacgac cgcaatgcag
      961 acccgggtcc tccagcgcta ccagcacagc tttcctccgc ggccccagga gctgcggggt
     1021 ccgcgctcct ctcgtgccct gcgcgcccgg cgccttcccc agtgagcgcg ggtccccagc
     1081 cctggtccag agcgctccgg acgtctctgc cttcccctcc ccctgcgaac tggccccggg
     1141 ggtggggttt ccctgtgcgc tcgcccccac ccctagtttg tgccctcccc tcccccgccc
     1201 gcccctatgt atgtgtcacc gcgcaccatt cccgcccacc tacctccccg cggtgccaga
     1261 ggggctcaca gagctgagga cgcgtgcagt accgcccaag gtccctcatt ctccgcgact
     1321 agcatctgac acatgtgccg aaggaactca agacaccttt ctgtatccct tccctccatc
     1381 gaccctgctc cgaaacccag ccagccagct accactgcgg ccaccgcccg aggggacttg
     1441 cggaggagac gccggcggga ggagggcttc gcagccccca cccccacccc cactcccagc
     1501 ccagagcatc cctgctgcag cgcagcaaga cccggggctg ccagccccca ggaatcccta
     1561 gctgcttagc agggataacg gactgaagtt cttggaggag cgagttcaac tcttcaaggt
     1621 aaagaaaggg ctccttctct ccagaggagc tctgtcccag ggcatctggg aatgctgctg
     1681 aggacgcgcg cctaagtgct gggggtggcg tgccttggtg cccacattga gtatgtgtgt
     1741 tggggactca ggacattagc tgcatgccca tggaagttga gcaaactcaa ctgctgcccc
     1801 gactcaaagt gaatgggaac acgtggaggg gctcctgctc agagtacaca gaattaagaa
     1861 agaccagagc tttttcaagt ttaaagtacc ggggcgctaa actgaagtgt agagcgagaa
     1921 acagttttaa agccaaggaa atgtgtgttc aaaccccact cccccccgca acccctcgtt
     1981 cctctgggcg ctccataagg ctttcatatt ctcttgggaa ggccaactga tcagagaatc
     2041 agcaaaatta tgtcgagggg gggcccgccg gggatccccc gggctgcaga gactcccagg
     2101 aggccaaggc agctggctgc acatactcag tttgaagctc cacatgttca agccccagga
     2161 aaagctgatg cctcaggtca agtccaaagg gaggagaaag tcagcgggaa ggccatcagg
     2221 gaagaagtct ctctccctcc acagagggtc cttttttgtt taaagccttc atgggggtgg
     2281 ctgtgaccca cccacactga aaagggaggc cattctaggt ctacagactc cagagtttat
     2341 gcaaaacccc tcacagatat agaagagagc ataaacctga gcatctatgg tccgcctgtc
     2401 acatatcact ggctacccca ggaactcatt aggatgagtc tccactggct aggagatggg
     2461 ggaaccagta caccagccat cttggaatta agctaagaaa ggtcgaaggt ttctgaggaa
     2521 aggattctta gtgaaaacac tgtccaaacc atgacaaccc gctgttgtgc aatgtgactt
     2581 cagagccttg ggcatgaatt ttatcacttt tttttcttcc tttaactatc ccgtgcttta
     2641 actgtccaaa taacatccct gttgtctctt ccagctgaac tctgtcgacg gtatcgataa
     2701 gcttggatcc ccagcttggg ggatcgaaag agcctgctaa agcaaaaaag aagtcaccat
     2761 gtcgtttact ttgaccaaca agaacgtgat tttcgttgcc ggtctgggag gcattggtct
     2821 ggacaccagc aaggagctgc tcaagcgcga tcccgtcgtt ttacaacgtc gtgactggga
     2881 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2941 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3001 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
     3061 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
     3121 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
     3181 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     3241 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     3301 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
     3361 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct
     3421 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
     3481 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
     3541 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     3601 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     3661 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
     3721 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     3781 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     3841 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     3901 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
     3961 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
     4021 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
     4081 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
     4141 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     4201 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
     4261 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
     4321 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
     4381 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
     4441 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
     4501 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
     4561 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
     4621 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
     4681 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
     4741 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
     4801 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
     4861 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
     4921 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
     4981 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
     5041 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
     5101 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
     5161 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
     5221 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
     5281 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
     5341 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
     5401 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
     5461 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
     5521 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
     5581 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
     5641 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
     5701 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
     5761 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
     5821 ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca
     5881 gttggtctgg tgtcagggga tcctcgccac catgaaaaag cctgaactca ccgcgacgtc
     5941 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     6001 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     6061 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     6121 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     6181 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     6241 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     6301 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     6361 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     6421 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     6481 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     6541 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     6601 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     6661 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     6721 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     6781 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6841 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6901 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgac ccgggactag
     6961 tcgacctgca gccaacacct acaagcacag gctacagaag cggagcatga gacccacacg
     7021 gaggagccgc cccagcatgg cccactgaga ggagcttcca caatgtgccc aggatgctga
     7081 gcccagcggc cagccatgct gaccgtgcat cccactcaca tggtgtcttc ttgtcgcttt
     7141 ttgtcatata aggaatccat taaagaagga aaaaaaagcc acattgtgtg tgccccactc
     7201 cccatatcac ccaaactgca tctaatttat ccaccaccat caccgccccc cataaaaaac
     7261 cgccaaagca aattcactgt aacccatgga ctgagtttag agacatttct ttcatttccc
     7321 accgtgcctt tccagggacc agtgcaggga cagggtgaga agagttaaat taaataaaga
     7381 agattatttt ttgttgttgc ttcctgactt tacccaagaa cagcacaatt gttggttact
     7441 tcacctccag ggagagctag gggccagagg tggaattgga aggagacagg gccagggaga
     7501 gaggagtagg gcagccacac agaccagagc tttaaggact cagaagaggc aaactatagc
     7561 ttagtgtgtt ggacaaaagg gatcgaggtc tgtgccatct atgtgggaag gagcctgggg
     7621 cagggggcag gctggccaaa gggaagtgaa gttgcctttc ctccagggcc cctctcctag
     7681 gaacctttct cctaggaacc tttcttctgg gtcaaccacc ctagcaggtg tcatttctcg
     7741 gctggactgg gtaggggcac ttccttccca ggagtgctcc gccgtcccct tccctctccc
     7801 caccccggga cggtgcaggc accagtgttc cgtgcaccta tttatatttt tgaaaactaa
     7861 agattatgat aattataata ataaagacat tggaagagat ctgctagctc gagaattcga
     7921 tatcaagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc
     7981 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8041 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8101 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8161 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8221 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8281 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8341 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcga taggctccgc ccccctgacg
     8401 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8461 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8521 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8581 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8641 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8701 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8761 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8821 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8941 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9001 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9061 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9121 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9181 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9241 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9301 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9361 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9421 atagtttgcg caacgttgtt ggcattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9481 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9541 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9601 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9661 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa taccgcgccc
     9721 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa tagtgtatga catagcagaa
     9781 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9841 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9901 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9961 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10021 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10081 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10141 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10201 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10261 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10321 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10381 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgacgcgccc
    10441 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    10501 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    10561 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagagcttta
    10621 cggcacctcg accgcaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
    10681 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10741 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    10801 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaatatt taacgcgaat
    10861 tttaacaaaa tattaacgtt tacaatttcc attcgccatt caggctgcgc aactgttggg
    10921 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10981 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    11041 ccagtgaatt gtaatacgac tcactata
//
